Berried females that are captured by participating fishermen are measured and the developmental stage of the eggs that they are carrying is recorded. Egg stages range from 1-4 and are determined by color as well as the presence or absence of an eye spot. The following pictures illustrate the four stages recorded. Below this field key are more detailed pictures of eggs at each stage taken under a microscope.
For our purposes, we record four egg stages instead of five, as it is difficult to distinguish between stages 2-4 in the field. Our distinctions are as follows: Stage 1: All black, Stage 2: Some eggs have a lighter half, Stage 3: Eggs have an eye spot, Stage 4: Eggs are brown.
*Helluy, S. and Beltz, B.S. 1991. Embryonic development of an American lobster (Homaurs americanus): Quantitative staging and characterization of an embryonic molt cycle. Biol. Bull..180: 355-371.
Stage 1: <25%
Stage 2: 25-50%
Stage 3: 50-75%
Stage 4: 75-100%.
Following dissection a pleopod is removed from the female for viewing under a dissecting microscope. Female lobsters develop cement glands in their pleopods prior to egg extrusion. It is believed that these glands become engorged with a sticky material that "cements" the eggs to the lobster's abdomen once they are extruded.
These cement glands are visible under the microscope and the stage of their development can be determined. They are milky white and fill in from the edges of the pleopod towards the center. The stages are determined by the percent of the surface covered by the glands.
Criteria are defined by Aiken, D.E. and Waddy, S.L. 1982. Cement Gland Development, Ovary Maturation, and Reproductive Cycles in the American Lobster Homarus americanus. Journal of Crustacean Biology 2(3): 315-327.
As female lobsters approach maturity the width of their adomen increases relative to their carapace length so that they will be better able to carry their eggs. The picture to directly to the right illustrates a widened abdomen relative to the narrow abdomen to the far right. The increase of this ratio (abdomen width/carapace length) in females relative to the constant ratio observed in males is an indication of the onset of maturity. See graph below.
A small amount of fluid is removed from the spermatheca of the female to check for the presence of a spermatophore. This sample is examined under a compound microscope (10x). Lobster sperm are non-motile and are therefore tail-less. However, they do have three antennae at the head end.